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KMID : 1098420200280010009
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Korean Journal of Medicinal Crop Science
2020 Volume.28 No. 1 p.9 ~ p.20
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N-Methyl-D-Aspartate (NMDA) Receptor Antagonistic Effect and Ginsenoside Content of Panax ginseng C. A. Meyer Cultivar Root Extracts
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Lee Seung-Eun
Kim Jang-Uk
Jeong Hyeon-Soo
Choi Je-Hun
Jee Yun-Jeong
Kim Hyung-Don
Jang Gwi-Yeong
Hyun Dong-Yun
Kim Dong-Hwi
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Abstract
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Background: Although a number of Panax ginseng cultivars have been developed by Korean researchers in recent years, there has been insufficient analysis of their beneficial properties. In this study, we sought to identify useful ginseng varieties as functional materials.
Methods and Results: We evaluated effects of root extracts of 10 ginseng cultivars (Cheongsun; CS, Chunpoong; CP, Gopoong; GP, Gumpoong; GMP, K1, Sunhyang; SH, Sunone; SO, Sunpoong; SP, Sunun; SU and Yunpoong; YP) against the inhibitory effects of nitric oxide (NO) and reactive oxygen species (ROS) production in mouse brain microglial BV2 cells, as well as the binding of N-methyl-D-aspartate receptor (NMDAR), a marker related to memory. Ginsenosides, such as 20 (S)-protopanaxadiols (PPDs), including ginsenoside-Rb1, -Rb2, -Rb3, -Rc, -Rd, and - Rg3 and 20 (S)-protopanaxatriols (PPTs) including -Re, -Rg1, and -Rg2 were analyzed by HPLC.
We observed that the cultivar GMP showed the highest inhibitory effect (60.8%) against NO production at 20 ㎍/㎖. Those cultivars showing the significantly highest inhibition effects against ROS at 20 ㎍/㎖ were K1 (57.3%), SP (54.5%), YP (53.1%), CP (51.7%), CS (50.9%) and SH (49.6%). At 50 ㎍/㎖, K1 showed the most potent inhibitory effect (51.2%) on NMDAR binding.
The total phenol content of SH (1.89 ㎎/g) and K1 (1.73 ㎎/g) were higher than those of the other cultivars, whereas in terms of PD/PT ratios, the values of CP (0.98), K1 (1.05) and SO (1.05) were lower than those of the other cultivars. On the basis of correlation coefficient (0.7064) between NMDAR inhibition and ONOO? scavenging activity.
Conclusions: The findings of this study indicate that the cultivars K1 and SH could be useful ginseng resources as
functional materials with favorable cognition-improving and antioxidative properties.
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KEYWORD
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Panax ginseng, Anti-oxidation, Anti-inflammation, N-methyl-D-aspartate Receptor, Total Phenol, Ginsenosides
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